anti-chicken secondary antibody donkey (polyclonal) chicken igg, conjugated to alexa fluor 488 #: 703-545-155  (Jackson Immuno)

Journal: Laboratory Animal Research

Article Title: The mesocortical dopaminergic system cannot explain hyperactivity in an animal model of attention deficit hyperactivity disorder (ADHD)- Spontaneously hypertensive rats (SHR)

doi: 10.1186/s42826-023-00172-5

Figure Lengend Snippet: List of antibodies and dilutions used in the study

Article Snippet: The sections were then transferred into an antibody cocktail that contained 1:300 monoclonal anti-DBH (Millipore Co. Temecula, CA, catalog no MAB308; 1:300) and 1:1000 chicken polyclonal anti-TH (Abcam, Cat# AB76442, 1:1000), 0.05% NaN 3, 0.3% Triton X-100, 1% BSA in PBS, and they were incubated at room temperature for 48 h. Then the sections were rinsed three times in PBS (5 min each), and incubated in a mixture of donkey anti-mouse secondary antibody conjugated with rhodamine (TRITC) (red, Jackson Immunoresearch Laboratories, Inc., West Grove, PA; catalog no: 715-025-151,1:500) and polyclonal donkey anti-chicken secondary antibody conjugated with Alexa Fluor 488 (green, Jackson Immunoresearch Laboratories, Inc., West Grove, PA, catalog no: 703–545-155, 1:500) in PBS for 2 h at room temperature in the dark.

Techniques: Plasmid Preparation